Portable Amplification and Quantification of DNA or RNA Fragments: Military Application

The amplification and quantification of DNA or RNA fragments is a key technique in molecular biology, used to identify genetic material associated with pathogens or specific conditions. In the military context, this capability takes on strategic importance, enabling early detection of biological threats in environments where access to conventional medical infrastructure is limited or non-existent. Traditionally, this process requires specialized equipment (PCR), trained personnel, and laboratory conditions. However, recent advances in molecular diagnostics have led to alternative methods—such as isothermal amplification (e.g., LAMP or RPA)—that can operate in field conditions without the need for complex thermal control or logistical resources. These technologies, integrated into portable, rugged, and automated devices, allow for high-precision diagnostics directly in the field. Their use in military operations enables rapid detection of pathogens (viruses, bacteria, biological agents) that could compromise troop health, as well as the genetic identification of deliberate biological threats. Furthermore, their ability to generate real-time quantitative results allows for immediate medical and operational decision-making, enhancing the response to infectious outbreaks, minimizing the risk of spread, and strengthening the biological security of deployed forces. These solutions transform molecular diagnostics into a tool for tactical health intelligence, crucial for maintaining operational readiness, force protection, and battlefield superiority.